Gaps between song files

I use iTunes on my Mac to play our rehearsal track that has each song split into about 7 files each. I need the files to play seamlessly with absolutely no gap. There is a tiny hiccup of a gap between each file which is unacceptable both for rehearsal and performance. Going to Settings>Playback doesn't give an option to create zero gap between the song files. Please help! Rehearsal begins tomorrow night!